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| 4孔腔室载玻片-无凹液面 |
| 产品型号：80446 |
| 产品品牌：ibidi |
| 产品规格： 15个/盒 |
- excellent phase contrast in the µ-slide 4 well ph
一个开放的四孔培养载玻片，带有一个特殊中间板，非常适用于相差和高端的荧光显微镜
整个培养孔具有优秀的相差-没有弯月面现象
一体化小室，既可用于细胞培养，也可用于显微镜成像
使用高分辨率显微镜可以通过玻璃片底部进行样品观察
没有盖玻片，不会泄露，用于快速，简单的免疫荧光。
货号 产品名称 规格（个/盒） 80446 µ-slide4孔ph 腔室载玻片，ibitreat底部处理 15 80442 µ-slide 4孔ph 腔室载玻片，collagen iv底部处理 15 80443 µ-slide 4孔ph 腔室载玻片，fibronectin底部处理 15 80444 µ-slide 4孔ph 腔室载玻片，poly-l-lysine底部处理 15 80445 µ-slide 4孔ph 腔室载玻片，poly-d-lysine底部处理 15 80441 µ-slide 4孔ph 腔室载玻片，无包被 15 80447 µ-slide 4孔ph 腔室载玻片，玻璃底 15
应用
细胞培养以及细胞培养物的显微镜观察。
转染分析
活细胞和固定细胞的免疫荧光显微镜观察
长时间段下活细胞成像
技术指标
孔数量 4 带盖子高度 10.8 mm 孔尺寸大小 21.2 x 11.0 x 3.0 每孔生成面积 2.2 cm² 每孔体积 700 µl 每孔生成面积 5.9 cm² 底部ibidi 标准底部
技术特点
开放式，两个独立的培养孔载玻片
每个培养孔上的中间板可以避免弯液面形成
从两侧缝隙很容易加液，无气泡形成，
适用于高端显微镜的优秀光学成像特质
兼容染色，固定
最佳细胞粘附的表面ibitreat
生物兼容性塑料生产，无胶水，不泄露
phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. when working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. in open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. this miscalibrates the phase rings, leading to poor contrast in the microscopic image.
µ-slide 4 well µ-slide 4 well ph
working with the µ-slide 4 well ph diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.
cross section through a well of the µ-slide 4 well ph with a transmitted light beam path
the illustration on the left shows the perturbing effect of a meniscus. light is refracted on the air-water-interface, leading to poor contrast in microscopy. only the small center part exhibits satisfying phase contrast.
working with the µ-slide 4 well ph diminishes the meniscus and increases the area of nicely contrasted cells. this nice contrast is due to the parallel beam path that is created by the plate.
comparison ph well versus standard well:
no meniscus effect in ph well
µ-slide 4 well ph
excellent phase contrast
excellent fluorescence microscopy
standard well
poor phase contrast
excellent fluorescence microscopy
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